standard cy5.5 set (xf141–2)  (Omega Optical)

Journal: BioMed Research International

Article Title: Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

doi: 10.1155/2016/6825712

Figure Lengend Snippet: Reaction scheme for the synthesis of Mab-Cy5.5.

Article Snippet: The fluorescence signals of the cells were imaged with an AxioSkop fluorescence microscope (ZEISS) equipped with Cy5.5 filter set and a charge-coupled camera (AxioCam MRC5, ZEISS).

Techniques:

Journal: BioMed Research International

Article Title: Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

doi: 10.1155/2016/6825712

Figure Lengend Snippet: In vitro binding characteristics of Mab-Cy5.5. NIRF images were obtained after Hela, HT-29, PANC-1, and RCC4 cells under hypoxia (a, c, e, and g) or under normoxia (b, d, f, and h) were incubated with 10 nM Mab-Cy5.5.

Article Snippet: The fluorescence signals of the cells were imaged with an AxioSkop fluorescence microscope (ZEISS) equipped with Cy5.5 filter set and a charge-coupled camera (AxioCam MRC5, ZEISS).

Techniques: In Vitro, Binding Assay, Incubation

Journal: BioMed Research International

Article Title: Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

doi: 10.1155/2016/6825712

Figure Lengend Snippet: In vitro binding characteristics of Mab-Cy5.5. RCC4 cells were incubated with (a) 10 nM Mab-Cy5.5 and 100 nM free Mab under hypoxia; (b) 10 nM Mab-Cy5.5 and 100 nM free Mab under normoxia; (c) 100 nM free Cy5.5 under hypoxia; (d) 100 nM free Cy5.5 under normoxia.

Article Snippet: The fluorescence signals of the cells were imaged with an AxioSkop fluorescence microscope (ZEISS) equipped with Cy5.5 filter set and a charge-coupled camera (AxioCam MRC5, ZEISS).

Techniques: In Vitro, Binding Assay, Incubation

Journal: BioMed Research International

Article Title: Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

doi: 10.1155/2016/6825712

Figure Lengend Snippet: In vivo fluorescence imaging of athymic mouse bearing HT-29 tumor xenografts at 1–72 h after injection of 0.25 nmol Mab-Cy5.5. The location of the tumor was indicated by an arrow.

Article Snippet: The fluorescence signals of the cells were imaged with an AxioSkop fluorescence microscope (ZEISS) equipped with Cy5.5 filter set and a charge-coupled camera (AxioCam MRC5, ZEISS).

Techniques: In Vivo, Fluorescence, Imaging, Injection

Journal: BioMed Research International

Article Title: Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

doi: 10.1155/2016/6825712

Figure Lengend Snippet: In vivo fluorescence imaging of athymic mouse bearing HT-29 tumor xenografts at 1–72 h after injection of 0.25 nmol Mab-Cy5.5 and 1.5 nmol free Mab. The location of the tumor was indicated by an arrow.

Article Snippet: The fluorescence signals of the cells were imaged with an AxioSkop fluorescence microscope (ZEISS) equipped with Cy5.5 filter set and a charge-coupled camera (AxioCam MRC5, ZEISS).

Techniques: In Vivo, Fluorescence, Imaging, Injection

Journal: BioMed Research International

Article Title: Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

doi: 10.1155/2016/6825712

Figure Lengend Snippet: Representative image of dissected organs of mouse in the experiment sacrificed at 72 h after injection of Mab-Cy5.5 (1, brain; 2, small intestine; 3, spleen; 4, liver; 5, lung; 6, kidney; 7, cecum; 8, stomach; 9, heart; 10, tumor; 11, bone; 12, bladder; 13, muscle).

Article Snippet: The fluorescence signals of the cells were imaged with an AxioSkop fluorescence microscope (ZEISS) equipped with Cy5.5 filter set and a charge-coupled camera (AxioCam MRC5, ZEISS).

Techniques: Injection

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Imaging NF-κB activity in a murine model of early stage diabetes

doi: 10.1096/fj.201801147R

Figure Lengend Snippet: A. Molecular model of the near-infrared ODN duplex probe (NIR-ODND) with a single NF-κB consensus binding site carrying a Cy5.5 fluorophore on one of the ODN strands. B-electrophoretic mobility (4–20% polyacrylamide gradient /TBE) of the NIR-ODND and single ODNs. ODND was labeled with either Cy5.5 (lane 3, red) or 800CW (lane 4, green). The migration of single strand NIR-fluorophore labeled ODNs serve as controls and are shown in lanes 1 and 2. C-electrophoretic mobility shift assay (EMSA, 10% PAGE): quantitative analysis of shifts using Cy5.5-labeled ODND (fluorescence emission – 700 nm) as a probe for activated NF-κB in pancreatic extracts collected on day 8. NIR-ODND was incubated in the presence of: lane 1- none; lane 2- HeLa nuclear extract (positive control); lane 3- islet nuclear extract-control; lane 4 - islet nuclear extract-LD-STZ; lane 5- islet cytoplasmic extract-control; lane 6- islet cytoplasmic extract-LD-STZ. The arrowhead points to the shifted ODN bands. D-quantification of fluorescence intensity of shifted bands due to NIR-ODND banding to the components of islet extracts. E-electrophoretic mobility shift assay for testing the specificity of NIR-ODND binding: lane 1- Cy5.5-labeled ODND, lane 2- HeLa nuclear extract; lane 3 – HeLa and excess of non-labeled ODND; lane 4: inactive HeLa extract; lane 5- LD-STZ treated islet extract; lane 6- LD-STZ treated islet extract and excess of non-labeled ODND; lane 7- inactive LD-STZ treated islet extract. For testing band supershift in the presence of anti- NF-κB p65 antibody Cy3-labeled ODND was analyzed on 7.5% PAGE gels: lane 8- ODND only, lane 9 - LD-STZ treated islet extract; lane 10 - LD-STZ treated islet extract and anti- NF-κB p65 antibody.

Article Snippet: Images were captured using Nikon TE2000-U inverted microscope equipped with a standard Cy5.5 set (XF141–2, Omega optical).

Techniques: Binding Assay, Labeling, Migration, Electrophoretic Mobility Shift Assay, Fluorescence, Incubation, Positive Control